Organization of acetylcholine containing structures in the cranial motor nuclei of the rhombencephalon of the pig

We explored the immunoreactivity of choline acetyltransferase (ChAT) in the cranial nerve motor nuclei of the porcine rhombencephalon to reveal the cholinergic nature of these regions.In our experiments we used an immunohistochemical method for the visualization of all acetylcholine-containing structures.All studied motor nuclei contained ChAT-posi

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Tackling heterogeneity: a leaf disc-based assay for the high-throughput screening of transient gene expression in tobacco.

Transient Agrobacterium-mediated gene expression assays for Nicotiana tabacum (N.tabacum) are frequently used because they facilitate the comparison of multiple expression constructs regarding their capacity for maximum recombinant protein production.However, for three model proteins, we found that recombinant protein accumulation (rpa) was signifi

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